StreakFree

By: Pentium Bioscience  09-12-2011

Cell lysates in SDS PAGE loading buffer are often sticky because of insoluble large molecules.  This causes two problems: 
1) Difficult to pipet;  
2) Leave streaks on the gels (as show in picture below, left lane).

StreakFree solves these two problems and produces excellent protein profiles (as show in picture below, right lane).

PAGE Streak-Free, 2mL 35.00
S10905-006 PAGE Streak-Free, 6mL 72.00


Instructios:

  1. After heating samples, shake well PAGE StreakFree and immediately add it to the sample at 10%(v/v).
    We suggest re-suspending a cell pellet from 1 ml of culture in 100μl of the PAGE running buffer and 100μl of 2x PAGE loading buffer; then add 20μl of StreakFree.
  2. Centrifuge 2 minutes before loading the supernant onto your gel.

other notes:


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