By: Pentium Bioscience  09-12-2011

Cell lysates in SDS PAGE loading buffer are often sticky because of insoluble large molecules.  This causes two problems: 
1) Difficult to pipet;  
2) Leave streaks on the gels (as show in picture below, left lane).

StreakFree solves these two problems and produces excellent protein profiles (as show in picture below, right lane).

PAGE Streak-Free, 2mL 35.00
S10905-006 PAGE Streak-Free, 6mL 72.00


  1. After heating samples, shake well PAGE StreakFree and immediately add it to the sample at 10%(v/v).
    We suggest re-suspending a cell pellet from 1 ml of culture in 100μl of the PAGE running buffer and 100μl of 2x PAGE loading buffer; then add 20μl of StreakFree.
  2. Centrifuge 2 minutes before loading the supernant onto your gel.

other notes:

Contact Pentium Bioscience

Email - none provided

Print this page

Other products and services from Pentium Bioscience



Discard the liquid gently; remove the last drop of liquid on the tube opening by touching the tube opening on a paper tower. Add 200 ?l of Solution B, tap and keep the tube at room temperature for 1 minute to dissolve the pellet completely. The resulting DNA pellet can be visible or not depending on the copy number of a plasmid. You finish the whole process only in 1 tube for each sample and label your tubes once.



It not only wastes your precious time, reagents and your sunny mood, but also leaves a nasty and toxic mess on bench for you to clean-up. Squeeze PrevoLeak about 1mm high into the space along the bottom between the two glass plates, after assembling your gel cassette. Even you have taken extraordinary care to make the glass plates and the spacers absolutely even.


Plus2 SDS PAGE Kit

You are no longer to read your recipe, to make fresh ammonia persulphate, to hold your breath from stinky TEMED, to remember which ingredients added, to worry which ingredients missed or added less, and so on. Separating gels come with 4 concentrations of 8%, 10%, 12% and 15% or YOUR choice of concentrations in 7 ml tubes. With our kits, to make a gel, you simply pick up a base tube and add 2 solutions to the tube.