Plus2 SDS PAGE Kit

By: Pentium Bioscience  09-12-2011
Keywords: tube

  • Stacking gels are 5% in microtubes.
  • Separating gels come with 4 concentrations of 8%, 10%, 12% and 15% or YOUR choice of  concentrations in 7 ml tubes.

With our kits, to make a gel, you simply pick up a base tube and add 2 solutions to the tube.  That is it! 

You are no longer to read your recipe, to make fresh ammonia persulphate, to hold your breath from stinky TEMED, to remember which ingredients added, to worry which ingredients missed or added less, and so on. 

Keywords: tube

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It not only wastes your precious time, reagents and your sunny mood, but also leaves a nasty and toxic mess on bench for you to clean-up. Squeeze PrevoLeak about 1mm high into the space along the bottom between the two glass plates, after assembling your gel cassette. Even you have taken extraordinary care to make the glass plates and the spacers absolutely even.



We suggest re-suspending a cell pellet from 1 ml of culture in 100?l of the PAGE running buffer and 100?l of 2x PAGE loading buffer. Cell lysates in SDS PAGE loading buffer are often sticky because of insoluble large molecules. After heating samples, shake well PAGE StreakFree and immediately add it to the sample at 10%. StreakFree solves these two problems and produces excellent protein profiles.