Support - Application Protocols

By: Fermentas  09-12-2011

Protein fixation with glutaraldehyde is required before staining the gel with Coomassie Brilliant Blue dye (PageBlue™ Protein Staining Solution, #R0571) or silver (PageSilver™ Silver Staining Kit, #K0681).

Note
Other compounds commonly used for protein fixation (e.g., acetic acid, isopropanol, ethanol, TCA) are not suitable; proteins will wash away during staining procedure.

Procedure

  1. Add 100 ml of deionized or distilled water to the gel and wash for 1 min with gentle agitation. Discard the wash.
  2. Add 50 ml of freshly prepared 5% glutaraldehyde solution (gel should be covered completely). Fix with gentle agitation for 30 min. Discard the solution.
  3. Add 100 ml of deionized or distilled water to the gel and wash for 5 minutes with gentle agitation. Discard the wash. Repeat this step twice. The gel is now ready for staining.

For detailed staining protocols using Fermentas PageBlue™ Protein Staining Solution or PageSilver™ Silver Staining Kit, refer to the product manuals.

Note
Proceed directly to the staining step in PageBlue™ Protein Staining Solution protocol and to sensitizing step in PageSilver ™ Silver Staining Kit protocol.