Hamilton Thorne - Products - XYClone - Transgenic Applications - Developmental Biology

By: Hamilton Thorne  09-12-2011

XYClone is for non-clinical research use only. hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.

All photos below are courtesy Dr. Jon Henry.

Before and after laser ablation of planarian photoreceptors

Before and after laser ablation of slipper snail daughter cell (micromere)

Before and after laser ablation of C. elegans egg cell

Crepidula fornicata: Successive stages in the death of the 4d mesentoblast cell following laser ablation


Other products and services from Hamilton Thorne

09-12-2011

Hamilton Thorne - Products - XYClone - Transgenic Applications - apps lah

The objective of this study was to determine if lipid segregation with or without zona pellucida cutting on post thawed in vitro produced bovine embryos would effect survivability and development. These results demonstrate that laser assisted hatching using the XYClone system does not inhibit in vitro embryo viability and development.


09-12-2011

Hamilton Thorne - Products - XYClone - Transgenic Applications - apps icm

Our objective was to develop a laser-based system for the isolation of the inner cell mass that can develop into hESC lines, thereby circumventing immunosurgery that utilizes animal products. With laser-assisted ICM dissection, we were able to obtain in standard culture conditions, supported by feeder cells, ESC colonies at a similar rate than intact blastocysts.


09-12-2011

Hamilton Thorne - Products - XYClone - Transgenic Applications - apps transgenic

The tetraploid complementation approach directly yields mice that are almost entirely derived from ES cells, but it is inefficient, works only with certain hybrid ES cell lines and suffers from nonspecific lethality and abnormalities, complicating phenotypic analyses.