Hamilton Thorne - Products - XYClone - Transgenic Applications - apps lah

By: Hamilton Thorne  09-12-2011
Keywords: Laser Assisted, Assisted Hatching, Laser Assisted Hatching,

XYClone is for non-clinical research use only. hESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations.

"We perform on farm embryo collection and transfer. We travel almost 75K miles a year and setup in the clients' barn or house. The microscope and laser system is packed in a travel case and setup every day. Once the system is setup, calibrated, and targeted, I have not had to adjust it again. We work in some very dusty and dirty places and I have not had one problem with the system. In addition, the clients greatly enjoy being able to see their embryos before transfer and they can watch the computer screen without disrupting the embryologist." Michael Werhman, Ph.D., Rocky Mountain Reproductive Service

NEW FROM IETS 2008 - Jan 6-8, 2008

S. MengesA, H. WeiB, D. FaberB, D. KraemerA, and C. LongA
ADepartment of Veterinary Physiology and Pharmacology, Texas A&M University, College Station, TX 77843, USA, BTransova Genetics, Sioux Center, IA 51250, USA

"These results demonstrate that laser assisted hatching using the XYClone system does not inhibit in vitro embryo viability and development. A significant difference was found in the 60 day pregnancy rate between control and cut embryos. This difference exhibits a large potential for this system to improve 60 day pregnancy rates of in-vitro produced embryos."

J.H. Pryor1, C.R. Looney1, S. Romo2, D.C. Kraemer3and C.R. Long3
1Ovagenix, Bryan, TX 77805; 2Facultad de Estudios Superiores Cuautitlan, UNAM, Mexico; 3Texas A&M University, Department of Veterinary Physiology and Pharmacology, College Station, TX 77843

The objective of this study was to determine if lipid segregation with or without zona pellucida cutting on post thawed in vitro produced bovine embryos would effect survivability and development.

CBCFLAH and LAH exhibited a significantly greater number of both total and live cells than Control (P<0.05)

PREVIOUS STUDIES

Increased Pregnancy Rate for Bovine Embryos

In an unpublished study, a 20% increase in pregnancy rate occurred when the XYClone was used for laser assisted hatching of bovine embryos.

Image below shows frozen / thawed bovine embryos before and after laser assisted hatching (courtesy of Michael E. Wehrman, Ph.D., Rocky Mountain Reproductive Services)

Keywords: Assisted Hatching, Laser Assisted, Laser Assisted Hatching,

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09-12-2011

Hamilton Thorne - Products - XYClone - Transgenic Applications - Developmental Biology

HESC research using the XYClone should be conducted in accordance with any applicable Federal, State or local laws and regulations. Before and after laser ablation of slipper snail daughter cell. Before and after laser ablation of planarian photoreceptors. XYClone is for non-clinical research use only.


09-12-2011

Hamilton Thorne - Products - XYClone - Transgenic Applications - apps icm

Our objective was to develop a laser-based system for the isolation of the inner cell mass that can develop into hESC lines, thereby circumventing immunosurgery that utilizes animal products. With laser-assisted ICM dissection, we were able to obtain in standard culture conditions, supported by feeder cells, ESC colonies at a similar rate than intact blastocysts.


09-12-2011

Hamilton Thorne - Products - XYClone - Transgenic Applications - apps transgenic

The tetraploid complementation approach directly yields mice that are almost entirely derived from ES cells, but it is inefficient, works only with certain hybrid ES cell lines and suffers from nonspecific lethality and abnormalities, complicating phenotypic analyses.