Custom TOPO® Adaptation Services

By: Genesys Design  09-12-2011

Practically any vector you choose can be adapted for TOPO® cloning of PCR products at high efficiencies. Our scientists will design the cloning strategy and review it with you, modify your vector by covalently attaching the topoisomerase I enzyme, and prepare the vector ends for either blunt or TA Cloning® vectors. If you choose, we can also prepare your vector for directional cloning of PCR products. Each vector is functionally tested in TOPO® cloning reactions to ensure guaranteed cloning efficiencies.

High-Throughput Compatible

Functionally tested TOPO®-adapted custom vectors are supplied in bulk, for a minimum of 500 reactions, so they are HTP compatible. In addition, you'll receive high-efficiency competent cells in bulk or in a 96-well format for HTP cloning. You won't have to gather additional reagents to complete your cloning projects—so you'll save even more time.


Other products and services from Genesys Design

09-12-2011

Gateway® Vector Construction and Cloning Services

You may have the Entry clonetransferred to one or more Gateway® Destination vectors to create expression clones suitable for immediate application in the corresponding expression system. Since the gene has been cloned into the pCMV•Sport 6.1 vector, it is flanked by the attB1 and attB2 Gateway® recombination sites. The gene is composed of an open reading frame, illustrated as the area between the initiating methionine and the stop codon.


09-12-2011

Custom Services

Our wide range of service capabilities leverage the quality, consistent products from our award winning brands such as Invitrogen, Applied Biosystems, Molecular Probes, Ambion, and Gibco. When your studies require specialized, reliable, high-quality solutions, count on our highly trained staff to ensure your most challenging research goals are met.


09-12-2011

Instrument and Qualification Services

That’s why we dedicate so much of our time and energy to keeping your precision lab instruments tuned, qualified, and running flawlessly. We know you need confidence in your results, and we’re confident that we can help you achieve that goal through our unsurpassed service. Results that tell a patient their prognosis, or change the way we understand our world. Your data isn’t just about charts and tables—it’s about results.


09-12-2011

Services

From custom manufacturing, business consulting and protocol development, to instrument repair and optimization – we draw upon our wide range of premium, high quality products such as Invitrogen, Applied Biosystems, Ambion, Molecular Probes and Gibco, to create a completely customized, scalable, efficient solution for your research.


09-12-2011

Cloning Services

Cloning can sometimes be difficult and time-consuming, especially if you need to create custom vectors, to handle many sequences at once, or to express proteins using multiple systems. With years of molecular biology experience and access to the most advanced cloning technologies, we provide everything from custom vector design to high-throughput protein expression.