Discovery Services

By: Signalchem  09-12-2011
Keywords: Protein, Enzymes, Protein Kinase

SignalChem scientists have gained an extensive experience in the development and production of cellular signaling proteins and enzymes. Our scientists have the capabilities to undertake the custom development of the client's desired protein and enzyme target as well as the expertise to develop the appropriate assay systems to assess the respective biological activity of the protein and enzyme target.

Our scientists have developed proprietary methodologies to produce large quantities of extremely pure recombinant enzymes that possess high specific activity without compromising the fidelity of the active site. In general, SignalChem's enzymes such as active protein kinases represent the full-length human clone and do not possess any activating mutations as these mutations do not occur

in vivo


In addition, our scientists have an extensive experience in developing the appropriate assay systems for any biological enzyme and protein targets. In particular, SignalChem's validated assay systems can be used in high throughput format to identify potent inhibitor compounds. The identified inhibitor compounds can be counter-screened against SignalChem's expanding panel of diverse enzymes such as protein kinase and protein phosphatase targets. Using these methodologies, SignalChem can identify potent leads as well as determine the selectivity profile of the compound for our clients.

To learn more about how SignalChem can increase your research efficiency in a cost effective manner,

with your specific requirements.

Keywords: Enzymes, Protein, Protein Kinase, Protein Kinases,

Other products and services from Signalchem


Active Phosphodiesterases

Inhibition of selective PDE isoforms, which raises the levels of intracellular cAMP and/or cGMP, induces apoptosis and cell cycle arrest in a broad spectrum of tumour cells and regulates the tumour microenvironment. PDEs are involved in various pathogenic mechanisms that lead to Alzheimer's disease which includes neuronal apoptosis, neurovascular dysfunction, neurotransmitter modulation, and impairment of neurogenesis.


Active Kinases

Targeting protein kinases by small molecule inhibitors or neutralizing antibodies has been extremely beneficial in the treatment of various human diseases such as cancer and more than 10 drugs targeting protein kinases have been approved while another 60 candidates are in clinical trials.


Active Phosphatases

Protein phosphatases regulate the phosphorylation state of many important signaling molecules and are a key regulatory component in signal transduction pathways involved in the control of cell growth, proliferation, differentiation and transformation. Cellular proteins and lipids undergo cycles of phosphorylation and dephosphorylation reactions as part of post translational modification in response to various stimuli such as growth factors.


Active Histone Deacetylases

Histone deacetylases or HDACs are a class of enzymes that belongs to the histone deacetylase/acuc/apha family (there are at least 11 family members) and catalyze the deacetylation of lysine residues in different proteins. Members of the class I HDAC family can deacetylase the histone N-terminal tail and represses transcription in large multiprotein complexes with transcriptional co-repressors.