Apollo 324 Library Prep System for Next gen Sequencing

By: Ame Bioscience  09-12-2011
Keywords: Lower Labor Costs

Apollo 324 System for Next-Gen Sequencing Library Preparation 

The Apollo 324™ is a benchtop system that automates next-generation sequence library preparation workflows
by using bead technology to execute high-performance isolation and purification of nucleic acids (DNA, RNA, plasmid and so on) and proteins. The system automates end-repair, A-tail addition, adapter ligation and final double-bead-based cleanup and size selection.

The Apollo 324 enables simultaneous processing of one to eight samples, with batch processing for 32 samples at a time. Up to eight samples can be prepared in approximately four hours, yielding products ready for amplification or direct sequencing, and a batch of 32 libraries can be created in less than eight hours.

IntegenX  are validated to optimize overall system performance.  are available for the Illumina® Genome Analyzer and HiSeq™ 2000 NGS systems, with other kits due shortly to automate sample preparation for a variety of applications and platforms.

Apollo 324 Benefits
  • High quality, consistent libraries in less time
  • Fewer manual tasks, reduced errors and lower labor costs
  • Dual temperature zones ensure enzyme and reaction stability
  • Patented magnetic bead capture system provides flexibility to extend to applications such as RNA-Seq and ChIP-Seq
  • Significantly reduced reagent costs
  • High quality, consistent results with Less reagents and lower cost

The Apollo 324 significantly reduces the most laborious aspects of the library preparation workflow, and requires only about an hour of manual effort for setup. The automated workflow eliminates errors introduced by manual methods while ensuring high quality and consistent DNA libraries. Typical results have low contaminants—you can even bypass PCR and take the samples directly to the sequencer.

Pre-PCR Electropherograms of E. coli libraries processed on the Apollo 324

Easy to Use Touchscreen Interface
The Apollo 324 System has an easy to use touchscreen interface that guides you through each step of loading samples and reagents for a run. You then just press the RUN button to start the protocol. Throughout the run, a progress monitor provides a detailed status of each step in the protocol.

Location of reagents, consumables and samples on the work surface


The touchscreen interface guides you through the setup

Keywords: Lower Labor Costs

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