DyLight® Dyes Antibodies - Antibody & Reagent Professionals

By: Abd Serotec  09-12-2011
Keywords: Flow Cytometry, Fluorescence Detection, Dye Laser,

DyLight® dyes give you outstanding immunofluorescence results in flow cytometry and microscopy.

  • More intense fluorescence and sensitivity than comparable spectral conjugates
  • Greater photostability than other fluorochromes
  • Good stability over pH 4 - 9
  • Ideal for immunofluorescence techniques
  • Compatible with all common fluorescence detection channels
  • Available for custom conjugation

DyLight dyes are excellent alternatives to Alexa Fluor®, CyDye® and LI-COR® fluorescent dyes. DyLight dyes provide intense fluorescence and sensitivity requiring less conjugated antibody to study your cells than FITC and RPE, saving you money.

They are primarily suitable for use in immunofluorescence techniques such as microscopy and flow cytometry, and may also be used in ELISA, Western blotting, high throughput screening and other array platforms.

Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.

We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice.

Dye Laser
line (nm)
Maximal absorbance (nm) Maximal emission (nm) Suggested laser Fluores- cence
DyLight® 405 400 405 420 Violet diode
DyLight® 488 493 488 518 Argon-Ion
DyLight® 549 550 549 568 Krypton-Argon
DyLight® 649 646 649 674 Krypton-Argon
DyLight® 680 682 680 715 Argon-Ion
DyLight® 750 752 750 778 Argon-Ion
DyLight® 800 770 800 794 Argon-Ion
  • Exceptionally resistant to photo-bleaching and is an ideal blue fluorophore for demanding fluorescence conditions
  • Equivalent to Alexa Fluor® 405 and Cascade blue dyes

DyLight 405 Dye: Absorption (black), Emission (blue)

  • Outperforms FITC and Cy2 with fluorescent brightness, photostability and pH stability
  • Equivalent to Alexa Fluor® 488, fluorescein and FITC dyes

DyLight® 488 Dye: Absorption (black), Emission (green)

  • Has a bright and more photostable fluorescence than Cy3 dye
  • Equivalent to Alexa Fluor® 546, Alexa Fluor® 555, Cy3 and TRITC dyes

DyLight® 549 Dye: Absorption (black), Emission (yellow)

  • DyLight 649 closely matches Cy5 spectral properties with brighter and more photostable fluorescence
  • Equivalent to Alexa Fluor® 647 and Cy5

DyLight® 649 Dye: Absorption (black), Emission (red)

  • DyLight 680 is a superior alternative to Cy5.5
  • Can be separated from Texas Red or Rhodamine
  • Equivalent to Alexa Fluor® 680, Cy5.5
  • Compatible for use in the LI-COR® Odyssey Infrared Imaging System

DyLight® 680 Dye: Absorption (black), Emission (brown, near IR)

  • Equivalent to Alexa Fluor® 750
  • Higher fluorescence and photostability than LI-COR Dyes
  • Compatible for use in the LI-COR® Odyssey Infrared Imaging System

DyLight® 750 Dye: Absorption (black), Emission (brown, near IR)

  • Outperforms IRDye® 800 with extremely low autofluorescence – ideal for in vivo imaging
  • Compatible for use in the LI-COR® Odyssey Infrared Imaging System

DyLight® 800 Dye: Absorption (black), Emission (brown, infrared)

We offer custom conjugations of these DyLight® dyes to any of our primary antibodies or to your antibody. Not all antibody suppliers can offer you this Custom Service, just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.

Alexa Fluor® is a trademark of Molecular Probes Inc, OR, USA.
CyDye® is a trademark of GE Healthcare.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
LI-COR® and IRDye® 800 are trademarks of LI-COR Biosciences.

Keywords: Dye Laser, Flow Cytometry, Fluorescence Detection,

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