Antibody Production Services - AbD Serotec

By: Abd Serotec  09-12-2011
Keywords: Antibody Production, Protein A, Purification Processes,

Efficient and economic in vitro antibody production

Whether you need a large amount of antibody for a special project, or in-house demand exceeds your capacity, AbD Serotec is here to help. We offer a wide range of cell culture techniques to provide you with the most cost effective option for your project. You provide your hybridoma* and we’ll produce your antibody for you. This saves you time and lab resources.

  • Static flask, spinner flask and hollow fiber bioreactor cultures to meet all your production needs – from 10 mg to 10 g
  • Antibody purity assessed by SDS-PAGE, HPLC or IEF
  • Lot-to-lot consistency and batch-tested certification
  • ISO 9001 quality systems for fully traceable procedures and products

Purification

Our proprietary purification processes are designed to maximize the yield, purity, and immunoreactivity of your antibodies. We offer protein G, protein A and ion exchange chromatography purification processes. The method selected is based on the antibody provided and/or customer requirements.

Quality Control

We test and validate all antibodies according to our ISO 9001-accredited design and production quality assurance system. Antibody purity is assessed by SDS-PAGE, HPLC, or IEF. Our comprehensive testing facility includes ELISA, flow cytometry, immunohistochemistry and Western blot.

Hybridoma Storage

Following in vitro antibody production, we are able to offer hybridoma storage in liquid nitrogen at our purpose-built facilities. We can prepare a certified master cell bank for you. This reduces your risk of cell line loss and provides you with the security of another certified master cell bank.

Azide-Free Antibodies

Ideal for functional studies, we now offer antibodies in an azide free format. If required we can measure the endotoxin level in your antibody preparation (< 10 EU/ml).

Freeze Drying

This process lyophilizes your antibody, which can prolong the shelf-life and improve the temperature stability of antibodies, especially those conjugated with RPE and APC. We are happy to advise you on the suitability of freeze drying for your particular project.

Confidentiality

We treat all data and material that you provide us as completely confidential, right down to your name and affiliation. A signed Confidential Disclosure Agreement is available on request.

Customer Satisfaction

We pride ourselves on our flexibility towards customers’ needs and the highest quality pre- and post-production technical support. Our friendly and knowledgeable staff aim to answer all enquiries within a day.


* IMPORTANT NOTE - MYCOPLASMA
AbD Serotec must receive satisfactory certification that your hybridoma cells are free from mycoplasma, and reserves the right to commission independent testing at its discretion before receiving culture.

Keywords: Antibody Production, Protein A, Purification Processes,

Other products and services from Abd Serotec

09-12-2011

Alexa Fluor ® Conjugates - AbD Serotec

We also offer conjugation of these Alexa Fluor®, including the tandem dyes PE-Alexa Fluor® 647, PE-Alexa Fluor® 750, and APC-Alexa Fluor® 750 to any of our primary antibody catalog products. Alexa Fluor® fluorescent conjugates give you outstanding results in flow cytometry and immunofluorescence / confocal microscopy. Potentially more intense in fluorescence than other UV light-excitable blue conjugates.


09-12-2011

DyLight® Dyes Antibodies - Antibody & Reagent Professionals

We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice. Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.


09-12-2011

Antibody Conjugation Services - AbD Serotec

Our proprietary purification processes have been developed to maximize the yield, purity, and immunoreactivity of your antibodies. We test and validate all antibodies according to our ISO 9001-accredited design and production quality assurance system. Reproducible antibody conjugation is essential for consistent experimental results. The method selected is based on the antibody provided and/or customer requirements.