Alexa Fluor ® Conjugates - AbD Serotec

By: Abd Serotec  09-12-2011
Keywords: Flow Cytometry, Fluorescence Detection

Alexa Fluor® fluorescent conjugates give you outstanding results in flow cytometry and immunofluorescence / confocal microscopy. Alexa Fluor® conjugates are:

  • Brighter and more fluorescent than comparable spectral conjugates
  • More photostable than other fluorochromes
  • Insensitive to changes in pH over a broad range
  • Ideal for multicolor applications due to minimal spectral overlap
  • Compatible with all common fluorescence detection channels

Key Alexa Fluor® Conjugates

We also offer conjugation of these Alexa Fluor®, including the tandem dyes PE-Alexa Fluor® 647, PE-Alexa Fluor® 750, and APC-Alexa Fluor® 750 to any of our primary antibody catalog products. Not all antibody suppliers can offer you this service; and this is just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.

Technical Features of Alexa Fluor® Conjugates

Fluorochrome

Maximal absorbance

Maximal emission

Flow cytometry

Confocal microscopy

Color

Common detection channels

Alexa Fluor ® 405

401

421

Yes

Yes

Blue

FL-4, FL-6, FL-9

Alexa Fluor ® 488

495

519

Yes

Yes

Green

FL-1

Alexa Fluor ® 647

650

665

Yes

Yes

Red

FL-4, FL-5, FL-6, FL-8

Alexa Fluor ® 700

702

723

Yes

Yes

InfraRed

Alexa Fluor® 405

  • The best blue fluorochrome available
  • Potentially more intense in fluorescence than other UV light-excitable blue conjugates
  • Excited by Blue Diode, Violet, or Krypton lasers
  • Usually detected in the same fluorescence channel as Cascade Blue
  • Minimal overlap with green fluorophores

Alexa Fluor® 488

  • The finest green fluorophore available
  • Excited by an Argon-Ion laser
  • Usually detected in the same fluorescence channel as FITC
  • Alternative for fluorescein conjugates (FITC, FAM) and Cy2
  • Brighter and more photostable than fluorescein

Alexa Fluor® 647

  • A very high quality red/purple dye
  • Replaces APC (allophycocyanin) or Cy5
  • Excited by Helium-Neon or Krypton-Neon lasers
  • Usually detected in the same fluorescence channel as APC
  • Total fluorescence significantly higher than Cy5 dyes
  • More photostable than DPXL-3, Cy5, and APC conjugates

Alexa Fluor® 700

  • A near infrared dye
  • Excited by xenon-arc lamp, far red diode lasers or dye-pumped lasers (675-700 nm)
  • Usually detected in the same fluorescence channel as APC
  • Brighter than Cy5, Cy5.5, Cy7, and similar dyes
  • More photostable than Cy5 and APC conjugates

All AbD Serotec products are backed by our Performance Guarantee and supported by unrivalled pre- and post-sales technical support, so you can trust AbD Serotec for all your Alexa Fluor® conjugated antibodies.

Alexa Fluor ® is a registered trademark of Molecular Probes Inc. OR, USA

Keywords: Flow Cytometry, Fluorescence Detection

Other products and services from Abd Serotec

09-12-2011

Antibody Production Services - AbD Serotec

Whether you need a large amount of antibody for a special project, or in-house demand exceeds your capacity, AbD Serotec is here to help. Following in vitro antibody production, we are able to offer hybridoma storage in liquid nitrogen at our purpose-built facilities. Our proprietary purification processes are designed to maximize the yield, purity, and immunoreactivity of your antibodies.


09-12-2011

DyLight® Dyes Antibodies - Antibody & Reagent Professionals

We currently offer the following DyLight® dyes conjugated to primary antibodies and secondary antibodies, and they are also available for custom conjugations to the antibody of your choice. Their absorption and emission wavelengths are compatible with popular fluorescence instrumentation, so you won’t have to change your preferred method of imaging.


09-12-2011

Antibody Conjugation Services - AbD Serotec

Our proprietary purification processes have been developed to maximize the yield, purity, and immunoreactivity of your antibodies. We test and validate all antibodies according to our ISO 9001-accredited design and production quality assurance system. Reproducible antibody conjugation is essential for consistent experimental results. The method selected is based on the antibody provided and/or customer requirements.