Alexa Fluor ® Conjugates - AbD Serotec

By: Abd Serotec  09-12-2011
Keywords: Flow Cytometry, Fluorescence Detection

Alexa Fluor® fluorescent conjugates give you outstanding results in flow cytometry and immunofluorescence / confocal microscopy. Alexa Fluor® conjugates are:

  • Brighter and more fluorescent than comparable spectral conjugates
  • More photostable than other fluorochromes
  • Insensitive to changes in pH over a broad range
  • Ideal for multicolor applications due to minimal spectral overlap
  • Compatible with all common fluorescence detection channels

Key Alexa Fluor® Conjugates

We also offer conjugation of these Alexa Fluor®, including the tandem dyes PE-Alexa Fluor® 647, PE-Alexa Fluor® 750, and APC-Alexa Fluor® 750 to any of our primary antibody catalog products. Not all antibody suppliers can offer you this service; and this is just one of the many ways AbD Serotec tries harder to meet your specific antibody needs.

Technical Features of Alexa Fluor® Conjugates


Maximal absorbance

Maximal emission

Flow cytometry

Confocal microscopy


Common detection channels

Alexa Fluor ® 405






FL-4, FL-6, FL-9

Alexa Fluor ® 488







Alexa Fluor ® 647






FL-4, FL-5, FL-6, FL-8

Alexa Fluor ® 700






Alexa Fluor® 405

  • The best blue fluorochrome available
  • Potentially more intense in fluorescence than other UV light-excitable blue conjugates
  • Excited by Blue Diode, Violet, or Krypton lasers
  • Usually detected in the same fluorescence channel as Cascade Blue
  • Minimal overlap with green fluorophores

Alexa Fluor® 488

  • The finest green fluorophore available
  • Excited by an Argon-Ion laser
  • Usually detected in the same fluorescence channel as FITC
  • Alternative for fluorescein conjugates (FITC, FAM) and Cy2
  • Brighter and more photostable than fluorescein

Alexa Fluor® 647

  • A very high quality red/purple dye
  • Replaces APC (allophycocyanin) or Cy5
  • Excited by Helium-Neon or Krypton-Neon lasers
  • Usually detected in the same fluorescence channel as APC
  • Total fluorescence significantly higher than Cy5 dyes
  • More photostable than DPXL-3, Cy5, and APC conjugates

Alexa Fluor® 700

  • A near infrared dye
  • Excited by xenon-arc lamp, far red diode lasers or dye-pumped lasers (675-700 nm)
  • Usually detected in the same fluorescence channel as APC
  • Brighter than Cy5, Cy5.5, Cy7, and similar dyes
  • More photostable than Cy5 and APC conjugates

All AbD Serotec products are backed by our Performance Guarantee and supported by unrivalled pre- and post-sales technical support, so you can trust AbD Serotec for all your Alexa Fluor® conjugated antibodies.

Alexa Fluor ® is a registered trademark of Molecular Probes Inc. OR, USA

Keywords: Flow Cytometry, Fluorescence Detection

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